Effects of nitric oxide on mitochondrial damage caused by exogenous calcium / 中国病理生理杂志

ABSTRACT

AIM: To study the effects of nitric oxide (NO) on mitochondrial damage caused by exogenous calcium. METHODS: Normal myocardial mitochondria were divided into three groups; L-arginine control group (CG), Ca 2+-damaged group (DG) and L-NAME-preserved group (PG). Mitochondria of all groups were incubated at 30℃ with reaction medium containing 20 ?mol/L EDTA, 100 ?mol/L CaCl 2 and 1 ?mol/L L-NAME with 100 ?mol/L CaCl 2 respectively. Then the NO- 2/NO- 3 contents, mitochondrial viability and membrane potential were investigated.RESULTS: The NO- 2/NO- 3 contents of DG was obviously higher than that of CG and PG, meanwhile, there was no obvious difference between CG and PG. Mitochondrial viability and membrane potential of DG were significantly lower than that of CG and PG, and negatively related to NO- 2/NO- 3 contents (r=-0.5297, P