Effect of ATP on proliferation and membrane protein expression in immortalized human fibroblasts / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of ATP on proliferation and membrane protein expression in immortalized human fibroblasts. METHODS: Applying the normal human fibroblast cell line TIG-7 and OUMS-36, the immortalized human fibroblast cell line KMST-6 and SUSM-1 to culture for 24 hours and 96 hours with the different concentrations of ATP,ADP and AMP, respectively. The number of cell alive, DNA synthesis and the expression of [ 32P]-ATP labeled proteins in the membrane fraction were also observed. RESULTS: After 96 hours culture, the proliferation inhibition in immortalized cells exposed to 0.4mmol/L ATP was 77%, while that of normal OUMS-36 cells treated with the same concentration of ATP was 41%; the DNA synthesis of the immortalized cells exposed to 0.4mmol/L ATP was greater suppressed than that of normal OUMS-36 cells. ADP was the only reagent tested that reduced the number of cells significantly(P