Detection of Rpsl Gene Mutation of Mycobacterium Tuberculosis by PCR-SSCP Silver Staining / 中国医师杂志
Journal of Chinese Physician
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-517412
ABSTRACT
Objective:
To study the value of detection of rpsl gene mutation in streptomycin(SM)-resistance in clinical isolates of mycobacterium tuberculosis by PCR-SSCP,and to expect to set up a rapid detective method of rpsl gene mutation in mycobacterium tuberculosis,Method Eighty seven specimens isolated from patients were detected by PCR-SSCP silver staining,and using strain H 37 Rv as a control,Results In 87 mycobacterium tuberculosis clinical isolates,the rpsl DNA fragments from 16 drug-susceptible isolates had no mutation in rpsL gene,48 of 62 sputum specimens showed rpsl gene mutation by PCR-SSCP,positive rate was 77 4%.Conclusions The rpsL gene mutation is an important molecular mechanism of M.tuberculosis resistance to SM.PCR-SSCP might become a rapid detecting method of SM-resistance of M.tuberculosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Chinese Physician
Year:
2001
Type:
Article
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