A Study on Detection of Lymph Node Micrometastasis by Using Monoclonal Anti-keratin Antibody in Patients with Colorectal Cancer / 中国医师杂志

ABSTRACT

Objective:To study the significance of detection of lymph node micrometastasis by immunohistochemical method with monoclonal anti-karatin antibody in patients with colorectal cancer.Methods:We examined 331 lymph nodes obtained from 47 colorectal cancer patients who underwent a curative operation and diagosed by using immunohistochemistry SB techniques.Monoclonal mouse anti-keratin antibodies(AE1,AE1/AE3,AE3)were used as the primary reagents to identify tumor cells both in tumor tissues and lymph nodes.Results:A total of 36 micrometastasis among 331 lymph nodes(11 9%) were found.Duke's C staging patients had more lymph nodes metastasis than that in Duke's B staging patients(P