Simultaneous Determination of Contents of Piperacillin and Tazobactam in a Preparation With RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a RP-HPLC method to simultaneously determine the contents of piperacillin and taz_obactam METHODS:Using ? Bondapak C18 column as fixed phase,a mixture of methanol and purified water(34 5∶65 5)as mobile phase,and caffeine as internal standard,detecting wavelength220nm,flow rate1 0ml/min RESULTS:The linear rangs of piperacillin and tazobactam were 40～200?g/ml and 5 0～25 0?g/ml respectively The average recovery and RSD (n=5) were (100 2?0 61)% for piperacillin and (99 03?0 90)% for tazobactam CONCLUSION:This method is rapid,simple and accurate