Activation of NF－? B Signaling Pathway in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus / 中华皮肤科杂志

ABSTRACT

Objective To detect the activation of NF－? B signaling pathway in peripheral blood mononuclear cells(PBMCs) of patients with systemic lupus erythematosus(SLE) and investigate its clinical significance. Methods Activation of NF－? B was detected by electrophoretic mobility shift assay( EMSA), the expression of I? B? protein and its phosphorylated products were detected by Western blot. Anti-dsDNA antibody, IgG and IgM in supernatant of PBMC culture were tested by ELISA. Results (1)Elevated activation of NF－? B in PBMC of SLE patients was found when compared with the controls(P