Roles of subretinal fluid on expression of bcl-2 oncoprotein of cultured human retinal pigment epithelial cells and fibroblast cells / 中华眼底病杂志

ABSTRACT

Objective To investigate the effect of subretinal fluid (SRF) with different grades of proliferative vitreoretinopathy (PVR) on bcl-2 oncoprotein expression in retinal pigment epithelium (RPE) cells and fibroblast (FB). Methods Using immunohistological staining technique and Western-blotting method to detect the expression of bcl-2 protein in RPE cells and FB under the stimulation of SRF. Results The expression levels of bcl-2 increased in both types of cells to a certain extent compared with those of the control group 4 hours after the cells subjected to SRF; 36 hours later, the expression levels of bcl-2 of experimental groups decreased more quickly than those of the control group,and the control group showed relatively higher bcl-2 protein levels at the end of observation. Conclusions SRF may stimulate the expression of bcl-2 in RPE cells and FB under culture at early stage, but accelarate the declining of bcl-2 protein levels a certain time after subjected to SRF.