~(15)N-glycine absorption in the colon of the short bowel rats / 中国普通外科杂志

ABSTRACT

Objective To investigate 15 N glycine absorption in the colon of the short bowel rats. Methods Rats undergoing resection of 80% small intestine and were treated by enteral nutrition for 21 days. The concentration of 15 N glycine in perfusate samples was measured by the technique of HPLC. The atom percent enrichment of 15 N glycine in blood plasma was measured by the technique of isotopic dilution method Results fter 3 hours, the colonic water absorption was 1?0 5 ml, 15 N glycine absorption was zero, and the atom percent enrichment was near zero in normal group, vs. (2 6?0 82) ml ( P