A Experimental Study on Treatment of Lung Cancer with Bacillus Calmette-Guerin Polysaccharides Nucleic Acid Combined with Chemotherapy / 中国医师杂志

ABSTRACT

Objective To investigate the mechanism of treatment of lung cancer with Bacillus Calmette-Guerin Polysaccharides nucleic acid (BCG-PSN) combined with Cyclophosphamide(Cy). Methods 130 mice were divided into three groups (A,B and C groups). Gruop A was further divided into four subgroups . Subgroup A-1 was given normal saline orally 0.25mg/10g?d -1 ; Subgroup A-2 was given levomisole PO. 0.25mg/10g?d -1 ; Subgroup A-3 was given BCG-PSN PO.0.325mg/10g?d -1 and subgroup A-4 was given BCG-PSN by intra-abdominal injection 0.25mg/10g?d -1 . Group B was given identical drugs with group A, after fifth day given 20% sheep red blood cell 0.2ml by intra-abdominal injection for five days . Gruop C divided into five subgroups;Subgroup C1-4 were given same drugs with group A,then given 0.4% Cy by subcutaneous injection 0.25mg/10g?d -1 from fifth day to tenth day.Subgroup C-5 was normal contral group, was given normal saline 0.25mg/10g?d -1 PO and subcutaneous injection 0.25mg/10g?d -1 .Results Oral administration alone or intra-abdominal injection in subgroup A-4 obviously enhanced the phagocytosis of macrophages.The subcutaneous injection of Cy could obviously decrease anti-tumour immunity, including activity of interleukin-2 (IL-2),natural killer(NK) cell, lyphocyte trasformation, and macrophages phagocytosis. BCG-PSN could increase and rehabilitate above anti-tumour immunological function. Conclusions Combinative treatment of BCG-PSN with Cy could remarkable enhance the immunocompetence.This experiment may provide a theoretical evidence for BCG-PSN combined with chemotheapeutic agents applied to treat the lung cancer.