Effects of oxidized HDL on the levels of MCP-1, ICAM-1 and free calcium in cultured human umbilical venous endothelial cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-517799
ABSTRACT
AIM:
To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration ([Ca 2+ ]i) level in cultured human umbilical venous endothelial cells(HUVECs).METHODS:
The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and [Ca 2+ ]i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS:
oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and [Ca 2+ ]i increased by 160%, 60% and 70% respectively compared with the control group ( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
Similar
MEDLINE
...
LILACS
LIS