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Effect of transfection of tumor necrosis factor ? gene and its mutants on tumorigenicity of H22 tumor cells in vivo / 中国病理生理杂志
Article in Zh | WPRIM | ID: wpr-518013
Responsible library: WPRO
ABSTRACT
AIM: To compare the tumorigenecity of H22 cells transfected with TNF-? gene and its mutants (secreted TNF-? mutant, S-TNFm, transmembrane TNF-? mutant, TM-TNFm and wild type of TNF-?, Wt-TNF) in vivo . METHODS: Three kinds of mouse liver cancer cell line H22 expressing TNF-? and its two mutants were mixed with untransfected H22 at different effector/target ratio separately. The growth of tumor was examined after injection of 2.5?10 5 (100 ?L) mixed H22 tumor cells into mice. The lymphocyte infiltration in the site of tumor and the expression of Fas on tumor cells were detected by immunohistochemistry. RESULTS: The tumorigenecity of H22 cells transfected with TNF-? gene and its mutants was significantly weakened ( P
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Pathophysiology Year: 2000 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Pathophysiology Year: 2000 Type: Article