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The protection of aminoguanidine,silymarin and anisodamine on growth and cytosolic free calcium changes of retinal capillary pericytes cultured in glycation products / 中华眼底病杂志
Chinese Journal of Ocular Fundus Diseases ; (6)2001.
Article in Chinese | WPRIM | ID: wpr-518052
ABSTRACT
Objective To investigate the protective effect of aminoguanidine(AG),silymarin (Sil) and anisodamine (Ani) on retinal capillary pericytes cultured in glycosylation products. Methods MTT cololrimetric assay, thymidine incorporating and fluorescent indicator fura 2 acetoxy methyl ester (Fura 2AM) were used to study the influence of AG,Sil and Ani on the growth,DNA synthesis,and cytosolic free calcium([Ca 2+ ]i)changes of pericytes cultured in the medium contained early glycation products (EGs) or advanced glycation end products (AGEs). Results Cultured in the medium contained EGs,the A value by MTT assayed and amount of thymidine incorporating in AG group and Sil group were obviously elevated than those of control group(P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Ocular Fundus Diseases Year: 2001 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Ocular Fundus Diseases Year: 2001 Type: Article