Cloning of human sperm protein(SP17) and expression in escherichia coli DH5? / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-518234
ABSTRACT
AIM:
To obtain GST fusion protein of hSP17 gene and construct the recombinant plasmid for expression in E. coli.METHODS:
Total fragment of hSP17 cDNA gene were amplified by RT-PCR, then subcoloned into pGEX-3b to generate recombinant hSP17/pGEX. Right orientation of insert are identified by restricted enzyme digestion. Transform the correct recombinant plasmid into the E. coli DH5a. The expression of fusion proteins hSP17-GST were induced by adding isopropylthiogalactoside (IPTG). RESULTS andCONCLUSION:
The recombinant plasmid hSP17/pGEX-3b could express effectively in E.coli and a high level of fusion protein hsp17-GST with the predicted molecular weight was detected.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS