Mechanisms of nitric oxide synthesis induced by human complement C5b-9complex in glomerular mesangial cells of rats / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-518243
ABSTRACT
AIM:
To explore the mechanism of the nitric oxide (NO) synthesis induced by human C5b-9 complex in glomerular mesangial cells(MC) of rats.METHODS:
The MC of rats were cultured and stimulated with human complement C5b-9 complex to induce TNF? and IL-1?. At the same time, several parameters related to NO synthesis were measured at 3 h, 6 h and 24 h after C5b-9 stimulation. The effects of monoclonal antibodies against TNF? and IL-1? on NO synthesis were examined in this system.RESULTS:
TNF? concentration in supernatant from MC in C5b-9 group was higher than that of control group at 6, 24 h after stimulation with C5b-9 complex and reversed by adding anti-TNF? McAb. C5b-9 complex didn't stimulate the release of IL-1? in same system. In addition, the expression of iNOS mRNA in MC was observed at 3 h after stimulation with C5b-9. Levels of iNOS mRNA expression and cGMP in MC and NO - 3/NO - 2 in supernatant from MC in C5b-9 group were higher than those in control group at 6, 24 h after C5b-9 stimulation, these changes were also reversed by adding monoclonal antibody against TNF?.CONCLUSION:
C5b-9 complex could induce iNOS mRNA expression at 3 h after C5b-9 stimulation, and the synthesis of NO at 6, 24 h was related to TNF? released from cultured MC of rats by C5b-9 complex to a certain extent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
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