Rapid Quantitation of Lamivudine in Mouse Serum and Tissue Homogenates by RP-HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-518307
ABSTRACT
OBJECTIVE:
To develop a rapid RP-HPLC quantitative determination of lamivudine(LAM) in mouse serum and tissue homogenatesMETHODS:
0 1ml of serum of tissue homogenates and 0 5ml of methanol were vortexed in a plastic tube for 30s and the mixture was then centrifuged at 15 000r/min for 10min 20?l of the resulting supernatant was directly injected and LAM was chromatographed on a reversed-phase Kromasil C column(150mm?4 6mm,5?m) using a mixture of phosphate buffer(pH6 8)and methanol(91∶9,v/v)and monitored at 270nmRESULTS:
The standard curves were linear over a range from 0 25?g/ml to 50?g/ml The minimal detectable drug concentration was 60ng/ml(S/N=3) The average extraction recovery of LAM was more than 88% The average methodological recoveries of LAM ranged from 97% to 100% The within-dayand between-day precisions were less than 10%CONCLUSION:
The assay is economic,simple,rapid and specific
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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