Effect of hepatocyte growth factor on migration and proliferation in cultured retinal pigment epithelial cells / 中华眼底病杂志
Chinese Journal of Ocular Fundus Diseases
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-518375
ABSTRACT
Objective To investigate the effect of HGF on proliferation and migration in cultured human RPE cells. Methods Human RPE cells cultured in serum-free medium were treated with HGF(1,2,10,50,100 ?g/L), and MTT assay was used to detect the growth of the cells; an in vitro wound healing model was used to count the number of cells that had entered the denudate area in RPE migration treated with HGF (1,2,10,50,100 ?g/L) after 20 h. Results HGF(10,50,100 ?g/L) increased proliferation rates of cultured human RPE (18 2 % to 34 8 %), and at a concentration of 50 ?g/L on day 3 HGF induced the maximal increase of proliferation( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Ocular Fundus Diseases
Year:
2000
Type:
Article
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