Immunohistochemical study of mast cell tryptase of the experimental amniotic fluid embolism/~△ / 中国法医学杂志

ABSTRACT

Objective To observe the number of pulmonary mast cell tryptase(MCT) after amniotic fluid embolism(AFE) in experimental rabbits.Method The experimental amniotic fluid embolism rabbits model was established by injecting the amniotic fluid(AF) or placental extracts mixed with amniotic fluid(PAF)into the marginal vein of the pregnant rabbits.Immunohistochemistry and microscopy were employed to observe the number of pulmonary MCT in AFE rabbits.Results A marked increase in MCT positive cells was observed in lungs in both AF and PAF groups(average cell number were 32 98 and 45 53respectively)as compared with the control group(average cell number was 11 25).Conclusion The results indicated that the number of MCT cells were marked increased in case of the AFE.