Inhibitory effect of anti-hCD154 McAb on immune response / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effects of anti-hCD154 McAb on inhibition of immune response. METHODS: The inhibitory effect of anti-hCD154 McAb on lymphocyte proliferation was evaluated by mixed lymphocyte reaction (MLR) and its effect on immune response was studied in severe combined immunodeficiency (SCID) mice reconstituted with human PBMC. RESULTS: (1) There were significant differences between experimental groups and control group in incorporation of -TdR into DNA (counts?min -1 ).Under dosages of 5 mg/L-50 mg/L, the incorporation of -TdR into DNA (counts?min -1 ) were decreased with dose increase. The value of counts?min -1 was peak when the dose was 100 mg/L.(2) The percentage of human T cell in SCID mice was lower in anti-hCD154-treatment groups compared with the control groups. (3) On day 3 after peripheral blood mononuclear cells(PBMC) grafted , human IgG were not detected by ELISA in all treatment groups. On day 7, 21, 31 after PBMC grafted, engrafted human PBMC produced human IgG in all treatment groups, but the mean amount of human IgG in SCID mice was markedly lower in the anti-hCD154-treated group compared with the control group. CONCLUSION: Our results show that anti-hCD154 McAb suppress human T cell proliferation and suppress human IgG production of human B cell transplanted in SCID mice.