Effects of TNF? and IL-10 on cytomegalovirus infection in human embryonic lung fibroblasts / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-518843
ABSTRACT
AIM:
To study effects of tumour necrosis factor alpha(TNF?) and interleukin-10(IL-10) on human cytomegalovirus AD169 (HCMV AD169) infection in human embryonic lung fibroblasts (HEL),and the ability of the infected HEL to produce TNF?. We have attempted to understand the effect of cytokines in the immune of HCMV infection.METHODS:
TNF??IL-10 were added separately with different concentrations before 24 h HCMV infection of HEL to study the effect of TNF? and IL-10 on multiplication of HCMV. HCMV was incubated with HEL, TNF? in culture supernatants were measured at 4, 24, 48, 72, 96 h postinfection. The level of TNF? was measured by enzyme-linked immunosorbent assay( ELISA).RESULTS:
The addition of TNF? with the concentration of 10-100 ?g/L or IL-10 with the concentration of 1-10 ?g/L before 24 h HCMV infection of HEL could remarkably inhibited the multiplication of HCMV in HEL. Level of TNF? didn't increase in infected cell supernatants.CONCLUSION:
TNF? and IL-10 play an important role in the immune of HCMV infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
Similar
MEDLINE
...
LILACS
LIS