Metallothionein inhibits homocysteine-induced proliferation of rat vascular smooth muscle cells / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS: VSMCs proliferation was measured by -TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by [ 109 Cd]-hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS: Hcy(10 -6 -10 -4 mmol/L) stimulated -TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, -TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold ( P