Studies on the expression of Cyclin D1 and apoptosis in the ER(-) breast cancer cell line MDA-MB-435s / 中国普通外科杂志

ABSTRACT

Objective To study the relationship between the Cyclind D1 and apoptosis in the ER(-) breast cancer cell line. Methods Apoptosis was induced by 1,25(OH) 2D 3 in the ER(-) breast cancer cell line. The cell hyperplasia was detected by MTT, cell cycle and apoptosis detected by flow cytometric, apoptosis cells determined quantitatively by TUNEL, the expression of Cyclin D1 detected by immunohistochemical method. Results In this study,cell cycle was arrested at G 0/G 1 phase. The result showed a possible negative correlation between the expression of Cyclin D1 and apoptisis in MDA MB 435s cell line. Conclusions Cyclin D1 may reflect the effect of treatment in ER(-) breast cancer, and may be a new marker to predict the prognosis of breast cancer?