Model of primary cultured neuron injury induced by D-galactose / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-519373
ABSTRACT
AIM:
To establish a model of primary cultured neuron injury induced by D-galactose for the research in Alzheimer's disease.METHODS:
Primary rat neurons cultured for 6 days were exposed to 50 mmol D-galactose for 72 h. The neural growth and neurite density were observed with HE stain and microscope, the neural metabolism rate and apoptosis rate were examined with MTT, immuno-enzyme assay and flow cytometry, respectively, and the aldose reductase mRNA expression was also detected with RT-PCR.RESULTS:
The neural growth and development in neurons treated with D-galactose was retarded, the neural metabolism rate decreased from 0.762?0 030( n= 33) to 0 543?0 064( n= 11)( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS