Determination of Aristolochic Acid A in Decoction of Caulis Aristolochiae Manshuriensis by RP-HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-519605
ABSTRACT
OBJECTIVE:
To establish a RP-HPLC method for the determination of aristolochic acid A in decoction of Caulis Aristolochiae ManshuriensisMETHODS:
The analytical column was Spherisorb ODS2 column(4 6mm?250mm,5?m) The mobile phase consisted of a mixture,methanol-water-acetic acid(70∶27∶1) The flow rate was 1 0ml/min The UV detection wavelength was 250nmRESULTS:
The linear range was 0 0 128?g~0 4 096?g(r=0 9 999) The regression equation was Y=4 553 7+5 388 319 3X The average recovery of aristolochic acid A was 97 33%(RSD=2 34%)CONCLUSION:
This method is simple,sensitive and accurate
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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