The Effect of Rb Protein Expression on the Clinical Response of the Colorectal Cancer Patients Perfused with 5-Fluorouracil Before Operation / 中国医师杂志

ABSTRACT

Objective To investigate the effect of Rb protein expression on the clinical response of the colorectal cancer patients perfused with 5-Fu before operation. Methods The expression of Rb protein in 34 cases of the colorectal cancer sample was determined by streptavidin-peroxidase(S-P) immunohistochemical method and the positive reactions were analysed by ICM-100 Cell DNA Picture Analysis System. The apoptotic rate of colorectal cancer cells was examined by the TUNEL staining. The chemosensistivity of the colorectal cancer cells were tested by the MTT colorimetric assay of agar culture. The clinical response of the colorectal cancer patients perfused with 5-Fu before operation was observed simultaneously. Results ⑴There was the negative correlation between the resistance index of the colorectal cancer cells to 5-Fu (IC50/10-1 peak concentration) and the apoptotic rate of the colorectal cancer cells induced by 5-Fu (?=-0.53,t=-4.91,P1.0 belongs to the group of resistance,