Effects of different doses of propofol on the pulmonary infrastructure after intestinal ischemia-reperfusion in rats / 中华麻醉学杂志

ABSTRACT

Objective To study the effects of different doses of propofol on the changes in lung Ca content and pulmonary ultrastructure caused by intestinal ischemia-reperfusion (IIR) induced lung injury. Methods Forty male SD rats weighing 200-250g were randomly divided into 5 groups of 8 rats each group I sham operation (control group) group II small intestinal ischemia-reperfusion; group III propofol 4 mg-kg-1-h-1; group IV propofol 8mg-kg-h-1; group V propofol 10 mg-kg-1-h-1. The rats were anesthetized with 3% pentobarbital 40mg-kg-1 ip. Right carotid artery and left internal jugular vein were cannulated for BP and pulmonary arteral pressure (PAP) monitoring and fluid and drug administration. The animals were tracheotomized and mechanically ventilated. PETCO2 was maintained at 4 .67- 5.60 kPa. Abdomen was opened. Superior mesenteric artery (SMA) was isolated and clamped for 60 min followed by 120min reperfusion. In group III , IV and V 10min before SMA occlusion was released different doses of propofol was continuously infused until the end of experiment. In group I and II isovolemic normal saline was infused instead of propofol. Lung Ca2+ content was measured and ultrastructure of the lung examined. Results The lung tissue Ca2+ content increased significantly after IIR in group II and III as compared with group I , IV and V ( P