Two - step PCR - SSP genotyping for HLA - A locus and its comparison with serological typing results / 中国法医学杂志
Chinese Journal of Forensic Medicine
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-520074
ABSTRACT
Objective To identify routine forensic samples by genotyping method for HLA - A locus. Method A two - step PCR - SSP method was established. The first step is amplification with a pair of primers specific for all HLA - A alleles, the second step is amplification with primers specific for HLA - A30, A31, A33 respectively, using the first step amplification product as template. Secondly amplified PCR products were genotyped by electro-phoresis. Results 100 blood stains with a serological typing result of HLA - A30, A31 and A33 were tested with this method. The discrepancy rate between serological and genetic typing was 29%. Seminal stains, salivary stains reserved for 2 years and blood stains reserved for 18 years in room temperature gave satisfactory results. Conclusions It is better to replace serological typing by genetic typing. A two - step PCR - SSP genotyping method can be applied to forensic samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Forensic Medicine
Year:
2000
Type:
Article
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