The polymorphism of mtDNA HVI and the application of multiplex amplification of two mitochondrial DNA region to the species and individual identification / 中国法医学杂志
Chinese Journal of Forensic Medicine
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-520075
ABSTRACT
Objective To discriminate the species and individual identification with mitochondrial DNA (mtD-NA) sequencing in forensic medicine practice. Methods The multiplex PCR of mtDNA loop - D high - variation region and cytochrome- b region were investigated. The PCR products were detected with silver- stain method,followed by analysis of the PCR products with fluorescence sequence technique. Results The presence of two bands (358bp,279bp ) indicated the samples were from human, while only one band of 358bp indicated nonhuman origin. The part of mitochondrial DNA loop - D high - variation region (15997 ~ 16236) from 131 unrelated individuals of Guangdong population were sequenced. In all of these samples there were 69 nucleotide variations and 67 haplo-types.There was 2.679 mutation sites on average per person. The polymorphism was 97.92% . Conclusion The methods described here are reliable and very useful in species and personal identification of degraded samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Forensic Medicine
Year:
2000
Type:
Article
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