Effect of Pteris Semipinnata 5F on Fibroblasts Proliferation and Collagen Synthesis in Systemic Scleroderma Skin / 中华皮肤科杂志

ABSTRACT

Objective To study the effect of herbal medicine Pteris semipinnata5F on the fibroblasts from systemic scleroderma(SSc)skin and explore new drug for SSc treatment.Methods The SSc skin fibroblasts were cultured as an experimental model and the effects of Pteris semipinnata5F were detected.The cell proliferation was determined by MTT assay,and the collagen production by photometric determination.The mRNA expression for typeⅠandⅢprocollagen was detected by RT-PCR.Results It was shown that Pteris semipinnata5F could not only significantly inhibit the proliferation of the cultured SSc skin fibroblasts,but also significantly reduce the soluble collagen content in the culture medium(P