Determination of Anthraquinone Free Derivatives From Chinese Herbal in Dog Serum by HPLC / 中国药房
China Pharmacy
;
(12)1991.
Article
in Chinese
| WPRIM
| ID: wpr-520345
ABSTRACT
OBJECTIVE:
To establish a HPLC method for determination of anthraquinone free derivatives from Chinese herbal in dog serumMETHODS:
With Hypersil BDS C18 column,the mobile phase was methanol-0 5% acetic acid(80∶20) The sample was extracted with methanol and dried at room temperature The residue was reconstituted by methanol and analyzed at 430nmRESULTS:
The five components can be separated well The linear ranges were Aloe-emodin(0 15~6 0?g/ml,r=0 9 996) Rhein(0 14~5 6?g/ml,r=0 9 982);Emodin(0 13~5 2?g/ml,r=0 9 990);Chrysophanol(0 2~8 0?g/ml,r=0 9 991);Physcion(0 1~4 0?g/ml,r=0 9 994)CONCLUSION:
This method can provide the basis for vivo analysis of anthraquinone free derivatives and bioavailability study of Rheum palmatum preparations
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
1991
Type:
Article
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