Study of the Mechanism of Cassia Obtusifolia L in Decreasing Blood-lipid / 中国药房

ABSTRACT

OBJECTIVE:To study the mechanism of Cassia obtusifolia L in decreasing blood-lipid.METHODS:Primary cultures of rat hepatocytes in vitro were used to determine the concentrations of proteins in hepatocytes by the method of Folin-phenol reagent and to assay the cholesterol synthesis from 14 C-acetate with liquid scintillation count.RESULTS:Ex?tract of Cassia obtusifolia L inhibited cholesterol synthesis from 14 C-acetate,but cassiaside B didn't affect cholesterol synthe?sis.CONCLUSION:The mechanism of extract of Cassia obtusifolia L in decreasing blood-lipid may result from inhibition of cholesterol synthesis,but inhibiting cholesterol synthesis may be not the main approach of cassiaside B in decreasing blood-lipid.