Effect of vitamin E on ox-LDL-induced monocyte/endothelium adhesion / 中国病理生理杂志

ABSTRACT

AIM: To observe the role of ox-LDL in rabbit endothelium/circulating monocyte adhesion in vitro and the effect of vitamin E. METHODS: Cultured rabbit's endothelial cells were incubated with different concentrations of ox-LDL, then incubated with rabbit's monocytes to observe cell's adhesion. With Northern blotting, endothelial VCAM-1 mRNA expression was measured. By using vitamin E incubation before ox-LDL, above steps were repeated to observe the effect of vitamin E. RESULTS: When ox-LDL concentrations were 2 5 mg/L, 5 mg/L, 10 mg/L, monocytes adhesive to endothelium per microscope field were 132 8?20 2, 350 0?37 2, 502 6?78 8, respectively They were all significantly higher than that of control group (51 2?7 7, P