The consensus oligonucleotide competitive binding with NF-?B inhibits TNF production / 中国病理生理杂志

ABSTRACT

AIM: To investigate whether a consensus oligonucleotide, which contains nuclear factor-kappa B(NF-?B) binding site, decreases TNF production stimulated by lipopolysaccharide(LPS). METHODS: The thiophosphoric acid modified oligonucleotides were transferred into rat peritoneal macrophages directly, TNF content in LPS-stimulated cell culture supernatant was determined by ELISA.RESULTS: Adding the consensus oligonucleotides with NF-?B binding site into macrophages markedly decreased TNF production following LPS stimulation. CONCLUSION: These results show that use of the consensus oligonucleotides with NF-?B binding site, which can combine with NF-?B, can block or decrease TNF production induced by LPS in macrophages.