Preliminary Studies on Hepatic Tissue Proteome of HBV Relative Hepatocellular Carcinoma by Two-Dimensional Gel Electrophoresis / 中国医师杂志
Journal of Chinese Physician
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-520843
ABSTRACT
Objective This study was designed to analyze the proteome differences between cancer tissue and surrounding-cancer tissue using Two-Dimensional Gel Electrophoresis (2-DE) in patients with HBV relative HCC.Methods Immobile phase pH gradients (IPGs) for isoelectric focusing of proteins were used as the first dimension,and SDS-polyacrglamide gel electrophoresis(PAGE) as the second dimension. The gels were stained by silver, scanned by ImageScanner, analyzed with ImageMast software.Results Average spots expressed in cancer tissue,cirrhosis tissue and chronic hepatitis tissue were significantly different(P
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chinese Physician
Year:
2001
Type:
Article
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