Effect of Leukocyte Depletion Filter During the Treatment of Canine Hemorrhagic Shock / 中国医师杂志

ABSTRACT

Objective To study the injurous effect of leukocytes on vital organs of hemorrhagic shock dog and role of leukocyte depletion filter in reducing the injury.Methods A canine model of hemorrhagic shock was established.12 male dogs were divided into blank group,whole blood transfusion group and leukocyte depletion group. Same amount of 0 9% physiological saline,whole blood of the canine itself and filtrated whole blood (i.e. lack of leukocytes) were back transfused respective correspondingly, changes in parameters such as white blood cells counting, LDH, MDA, GOT and the pathological changes in heart, lung, liver and kidney were detected among different treatment groups.Results Higher level of WBC,LDH, AST and MDA were observed in the group with whole blood transfused than in the group of leukocyte-depletion transfused. The pathological changes in liver and kidney indicated more serious injury happened in whole blood group than in the leukocyte-depletion group.Conclusion The Leukocyte depletion filter in itself can definitely alleviate the damages in the canine vital organs when reperfusion is inevitable as a result of hemorrhagic shock.