The Therapeutic Mechanism of Dexamethasone in Hyperoxia-induced Lung Injury / 中华围产医学杂志

ABSTRACT

Objective To investigate the therapeutic mechanism of dexamethasone in hyperoxia-induced lung injury. Methods Use bronchoalveolar lavage (BAL) method to gain alveolar macrophages (AM) from newborn SD rats. AM were adherence purified for 24 hours, then randomly assigned to four groups Ⅰ. hyperoxia group, Ⅱ. hyperoxia plus LPS group, Ⅲ. hyperoxia plus dexamethasone group, Ⅳ. hyperoxia plus LPS plus dexamethasone group. Every group contains 7 samples. After cultured for 48 hours, supernatants were harvested. L-lactate dehydrogenase (LDH) activity? hydrogen peroxide (H 2O 2) and IL-8 contents of supernatants were examined in all groups. Results (1)48 h after culture, the content of IL-8 in groupⅠandⅡwas (46?15)pg/ml?(145?27)pg/ml respectively, in groupⅢandⅣwas(29?4)pg/ml?(39?8)pg/ml respectively, IL-8 content was decreased in group Ⅲ and Ⅳcompared with group Ⅰand Ⅱ(P