Determination of the Content of Puerarin in Compound Rhizoma Coptidis Graininess by HPLC / 中国药房
China Pharmacy
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-521002
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:To establish a method for determination of the content of puerarin in compound Rhizoma Coptidis graininess METHODS:HPLC method was used The samples were separated by C18 (4 6mm?250mm,5?m) column with the mobile phase of methanol-water(30∶70) The detection wavelength was set at 250nm Flow rate was 1 0ml/min Column temperature was 30℃ RESULTS:The linear ranges of puerarin was 0 312?g~1 560?g The average recovery was 99 2%,RSD=2 08%(n=5) CONCLUSION:The method is sensitive,rapid and accurate and suitable for the determination of puerarin in compound Rhizoma Coptidis graininess
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2001
Type:
Article