Effects of Arg-Gly-Asp-Ser tetrapeptide on proliferation and apoptosis of hepatic stellate cells in vitro / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effects of Arg-Gly-Asp-Ser (RGDS) tetrapeptide on proliferation,apoptosis and caspase 3 expression in FN-stimulated HSCs in vitro. METHODS:[ 3H]-thymidine incorporation,Annexin-V/Propidium Iodide double-labeled flow cytometry(FCM),TUNEL,scanning electron microscope and transmission electron microscopy were employed to estimate the influence of RGDS on proliferation and apoptosis of HSCs. The adhesion rates were observed by toluidine blue colorimetric assay. The expression of caspase-3 protein was detected by FCM. RESULTS:①Compared with control and FN groups,RGDS tetrapeptide at concentrations of 25 mg?L -1 ,50 mg?L -1 and 100 mg?L2 1 inhibited the proliferation of HSCs ( P