The Time-Effect Study of Two Animal Models of Hepatic Fibrosis / 中国医师杂志

ABSTRACT

Objective To analyze the differences of time-effect and pathologic feature in the course of development of liver fibrosis between the two kinds of hepatic fibrotic animal models, which were made by CCl 4 and pig serum (PS) methods.Methods Rat models of liver fibrosis were made by using CCl 4 and PS ten week methods.At 6,10,14 and 20 weeks of model making respectively,pathological features of liver fibrosis were observed and analyzed by Masson staining and computerized image analyzing system.Results In CCl 4-induced model,fibrotic pseudolobuli developed at 6 weeks during model making, became typical at 10 weeks, and got stable thereafter.Ballooning degeneration of liver cells was outstanding,and paralleled with model making course.In PS-induced model, fibrosis occurred at 10 weeks during model making, and still developed though the model making was stopped,but ballooning degeneration of liver cells was not presented.Conclusions There was some different in time-effect relation and pathologic feature of liver fibrosis between the two methods of model making. This indicated that different mechanisms were involved in the pathogenesis of liver fibrosis. The two methods of model making should be combined to study of liver fibrosis.The prophylactic and treatment by anti-fibrotic drugs should last no less than 14 weeks.