Effect of adenosine on myocardial expression of TNF-?, IL-6 mRNA in rats with pneumonia / 中国病理生理杂志

ABSTRACT

AIM: To observe the expression of TNF-?, I L-6 mRNA in injured myocardium caused by infectious pneumonia and the effects of exogenous adenosine.METHODS: Fifty rats were divided into five experimental groups at random. The model of pneumonia was replicated by the inj ection of staphylococcus aureus into the windpipe of rats. Adenosine-treated ra ts (A,B and C group) received daily injection of adenosine at different dosages (50, 100 and 150 ?g?kg -1 ?min -1 for 90 min) for 3 days. All rat s were killed on the fifth day. Pathological examination of myocardium were don e and TNF-?, IL-6 mRNA expression were detected by reverse transcription poly m erase chain reaction (RT-PCR). RESULTS: (1) Significant increa se in TNF-?, IL-6 mRNA expression was observed in myocardium of pneumonic rats compared with control group ( P0 05). IL-6 mRNA expression in adenosine-treated C group was lower than that in adenosine-treated B group ( P