Effect of tea-polyphenols on the activation of NF-?B an d the expression of TGF-?_1 mRNA in THP-1 cells incubated with VLDL, LDL or ox-LDL / 中国病理生理杂志

ABSTRACT

AIM: To investigate the effect of tea-polyphe nols (TP) on the activation of NF-?B and the expression of TGF-? 1 mRNA in TH P -1 cells (a human acute monocytic leukemia cell line). METHODS: TH P-1 cells were incubated with the different concentrations of TP, VLDL, LDL or o x-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-?B was detected with immunohistochemistry technique, the positive index of the TGF-? 1 mRNA ex p ression was detected by hybridization in situ, and accumulation of total cholest erol (TC) in cells incubated with 0 4-40 ?g/L TP was determined with oxidase a ssay. RESULTS: The nuclear malposition rate of NF-?B, the posit ive index of the TGF-? 1 mRNA expression and TC in THP-1 cells incubated with 0 4-40 ?g/L of TP were lower than those with 0 ?g/L of TP in TP-V group, TP-L group and TP-O ( P 0.05). CONCLUSION: TP inhibited the activation of NF-?B, the expressio n of TGF-? 1 mRNA and the foam cell formation in the mono-macrophage.