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Regulation by reactive oxygen species of matrix metalloproteinase-1,3 and tissue inhibitor of matrix metalloproteinase-1 in smooth muscle cells / 中国病理生理杂志
Article in Zh | WPRIM | ID: wpr-521579
Responsible library: WPRO
ABSTRACT
AIM: To understand whether reactive oxygen species promote the rupture of atherosclerotic plaques by regulating the balance of matrix metalloproteinase-1,3 (MMP-1,3) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in smooth muscle cells. METHODS: Aortic smooth muscle cells from 4-6months-healthy abortive fetuses were incubated for 24 hours with xanthine (100 ?mol/L) and xanthine oxidase (5 U/L) in vitro . MMP-1,3 and TIMP-1 in the concentrated culture media were measured by Western blotting ( n =3 independent experiments). RESULTS: Incubation with xanthine/xanthine oxdiase decreased the amount of MMP-1 in the aortic smooth muscle cells (21.2%?5.5% of the control group),and pro-MMP-1 was activated completely. Reactive oxygen species (ROS) also activated pro-MMP-3,and increased the production of MMP-3 in the aortic smooth muscle cells. On the other hand,ROS inhibited the production of TIMP-1 in the aortic smooth muscle cells. CONCLUSION: It is complicated that ROS regulates the balance of MMPs and TIMPs. ROS may contribute to matrix degradation and the rupture in the atherosclerotic plaques.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Pathophysiology Year: 2000 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Pathophysiology Year: 2000 Type: Article