Establishment of orthotopic implantation tumor and metastasis model of human colon cancer in nude mice / 中国普通外科杂志

ABSTRACT

Objective To establish an orthotopic implantation and metastasis model of human colon cancer in nude mice. Methods Tumor cell line SW1116 of human colon adenocarcinoma was inoculated subcutaneously into nude mice to develop implantation tumor.Histologically intact tumor tissue was then harvested and implanted to the colon wall of nude mice to set up a model similar to human colon cancer.The formation of implanted tumor rate, local tumor growth characteristics,and metastasis rates were examined. Results A 100% lymphatic metastasis rate was obtained in this model. The incidences of local lymphatic metastasis, peritoneal and liver metastases were 100%, 91.7% and 75.0% respectively.Emacication and exhaustion of the nude mice were presented in late stage of the experimentation. The median survival time of the tumor-bearing nude mice was 10 weeks. Conclusions The orthotopic implantation tumor and metastasis model provide useful tools for the study of mechanism of metastasis and its treatment of human colon cancer.