Determination of the Contents of Geniposide in Gardenia Jasminoides Ellis and Its Preparations by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a RP-HPLC method for the assay of geniposide in Gardenia jasminoides Ellis and its preparations.METHODS:The determination was carried out with RP-HPLC on Kromasil C 18 column(4.6mm?250mm,5?m)with acetonitrile-water(10∶90)as the mobile phase,and the geniposide was detected at a UV-wavelength of238nm.RESULTS:The linear range of geniposide was0.596?g～2.98?g(r=0.9997).It was found that the contents of geni?poside in Gardenia jasminoides Ellis,its fruit,peel,seed,Jiaweixiaoyao pill and Qingkailing injection were41.8mg/g,12.3mg/g,67.3mg/g,4.26mg/g and0.316mg/ml,respectively.The average recoveries of geniposide in Gardenia jasminoides Ellis,Ji?aweixiaoyao pill and Qingkailing injection were99.35%,99.51%and99.57%and the relative standard deviations(RSD)were0.52%,0.72%and0.73%,respectively.CONCLUSION:The method is simple,accurate,sensitive and stable.It can be used for the determination of geniposide in Gardenia jasminoides Ellis and its preparations.