Determination of the Concentration of Propofol in Plasma by RP-HPLC with Fluorescence Detection / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-521747
ABSTRACT
OBJECTIVE:
To build up a method for the determination of propofol in plasma by RP-HPLC with fluores?cence detection.METHODS:
The separation was performed on a reversed-phase Zorbax Eclipse XDB-C 18 column(150mm?4.6mm,5?m)with a mobile phase consisting of methanol-acetonitrile-0.005mol/L sodium acetate buffer(pH4.0)(55∶20∶25,V/V).Propofol was extracted from plasma and dissolved in the mobile phase then detected at276/310nm.RESULTS:
The calibration curve had the fine linearity in the concentration range of0.015625~8?g/ml(r=0.9998).The limit of detection(LOD)was1ng/ml(S/N ratio=3),the limit of quantification(LOQ)was10ng/ml.The absolute recovery was89.33%~93.37%,the relative recovery was97.75%~103.31%.The within-day and between-day precision(RSD%)was1.38%~5.02%and4.45%~9.056%respectively.CONCLUSION:
The method is simple,stable and highly sensitive and can meet with the research of clinical pharmacokinetics.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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