The Effects of Inhibiting Ubiquitin-proteasome Pathway on DNA Synthesis and Cell Cycle in Gastric Cancer Cell Line SGC-7901 / 中国医师杂志
Journal of Chinese Physician
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-522053
ABSTRACT
Objective To investigate the effects of inhibiting ubiquitin-proteasome pathway(UPP) on DNA synthesis and cell cycle in gastric cancer cell line SGC-7901. Methods The SGC-7901 cells were treated with MG-132, a specific inhibitor of UPP. The effect of MG-132 on cells DNA synthesis was assayed by 3 H-thymidine( 3H-TdR)incorporation. Cell cycle was analysed by flow cytometry(FCM). Results After treated with MG-132, DNA synthesis of SGC-7901 cells was significantly inhibited, the ratio of G 0/G 1 phase cells increased, and the ratio of G 2/M phase and S phase cells decreased. Conclusions MG-132 can significantly inhibit DNA synthesis of gastric cancer cells SGC-7901 and induce G 1 blocking. The data indicated that inhibiting UPP may be a new strategy to treat gastric cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chinese Physician
Year:
2001
Type:
Article
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