Effects of stellate ganglion block on the heat shock protein 70 expression in hippocampus and temporal lobe following global cerebral ischemia-reperfusion injury in rabbits / 中华麻醉学杂志

ABSTRACT

Objective To investigate the effects of unilateral stellate ganglion block (SGB) on the expression of heat shock protein 70(HSP70) in bilateral hippocampus and temporal lobe induced by global cerebral ischemia-reperfusion in rabbits. Methods Twenty-eight healthy Japanese long-ear rabbits of either sex weighing 2.5-3.0 kg were anesthetized with intravenous 1.5% pentobarbital, intubated and mechanically ventilated. Global cerebral ischemia was produced by clamping bilateral external and internal carotid arteries and vertebral arteries for 10 min. Global cerebral ischemia was confirmed by blanching of lips and conjunctival, respiratory and EGG changes. The arteries were then declamped for reperfusion. The animals were randomly divided into 4 equal groups ( n = 7) SGB group, NS control group, blank control group and sham operation group. In SGB group left stellate ganglion was blocked by 0.25% bupivacaine 0.5 ml injected through an epidural catheter with its tip placed close to left stellate ganglion when reperfusion was started followed by continuous infusion of 0.25 % bupivacaine at a rate of 0.5 ml ? h -1 . SGB was confirmed by ptosis and miosis. In NS control group normal saline was used instead of 0.25 % bupivacaine. In blank control group no medication was give. In sham operation group the six arteries were exposed but not occluded. The HSP70 expression in the neurons in hippocampus CA1 section and cortex of temporal lobe was detected by immunohistochemistry using an antibody specific for HSP70 30h after reperfusion was started. Results HSP 70 expression in bilateral hippocampus CA1 section and cortex of temporal lobe was significantly lower in SGB group compared with the two control groups ( P 0.05) . Conclusion Left SGB can depress the over-expression of HSP 70 in bilateral hippocampus and temporal lobe induced by global cerebral ischemia-reperfusion.