Effect of 17?-estradiol on the BMPR-ⅠA,ⅠB gene expression in bone marrow stromal cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-522136
ABSTRACT
AIM:
To investigate the effects of 17?-estradiol (E_2) on the gene expression of typeⅠA and typeⅠB bone morphogenetic protein receptor (BMPR-ⅠA,ⅠB) in rat bone marrow stromal cells exposured to the differentiation medium and to elucidate the effects of E_2 on osteoblastogenesis.METHODS:
Adherent bone marrow stromal cells were cultured in differentiation medium containing DEX(10 -7 mol?L -1 ) and 1,25(OH)_2D_3 (10 -9 mol?L -1 ) and different concentrations of E_2. The gene expression of BMPR-ⅠA,ⅠB was quantified by semiquantitative RT-PCR.RESULTS:
E_2 evidently inhibited the expression of BMPR-ⅠA mRNA in bone marrow stromal cells.The suppression was dose-dependent. When examined under various concentrations of E_2 (0-10 -6 mol?L -1 ),the expression of BMPR-ⅠA mRNA were decreased from (25.7?2.5)% to(16.3?1.5)%( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
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