Effects of LPS,TNF-? and IL-1? on cyclooxygenase-2 expression in human umbilical vein endothelial cells / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-522157
ABSTRACT
AIM:
To explore effects of LPS,TNF-?,IL-1? on cyclooxygenase-2(COX-2) expression and prostaglandins(PGs) production in human umbilical vein endothelial cells (HUVEC).METHODS:
HUVEC were stimulated with lipopolysaccharide(LPS),TNF-?,IL-1? for 24 hours. Using in situ hybridization,reverse-transcription polymerase chain reaction and immunohistochemistry, COX-2 expression in both mRNA and protein level were observed and prostaglandins in culture medium were measured.RESULTS:
Resting HUVEC expressed a little COX-2 mRNA. Expression of mRNA and protein of cyclooxygenase-2 increased significantly post-stimulation with LPS,TNF-?,IL-1? and PGs raised markedly at the same time.CONCLUSIONS:
Resting HUVEC expressed a little COX-2 mRNA, inflammatory stimuli induced COX-2 superexpression and PGs production. These results suggested that endothelial cells could involve in inflammation by controlling of COX-2.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
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