Protective effect of HSP_70 on injuried myocardial cells induced by H_2O_2 / 中国病理生理杂志

ABSTRACT

AIM: To investigate the role of heat-shock protein 70(HSP_(70)) in the protection of myocardial cells against ischemic injury. METHODS: Myocardial cells were cultured in vitro. HSP_(70) was induced by hyperthermia.H_2O_2-injured myocardial cells were divided into different groups. Flow cytometry, DNA Ladder and biochemistry methods were employed to observe the myocardial cells of different groups. RESULTS: Immunohistochemistry showed hyperthermia induced the up-regulation of HSP_(70) in myocardial cells. Apoptotic rate, activity analysis of cytochrome C and succinic dehydrogenase in H_2O_2-injuried and HSP_(70)-protected groups were obviously different. Electron micrograph shomed hyperthermia alliviated myocardial cell injury induced by H_2O_2. CONCLUSION: HSP_(70) delays apoptosis and protects against H_2O_2-induced myocardial cell injury.