Effects of ?_3 integrin on adhesion and migration of vascular smooth muscle cells induced by growth factor / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-522301
ABSTRACT
AIM:
To investigate the effect of platelet-derived growth factor (PDGF) on ?_3 integrin gene expression and the role of ?_3 integrin on adhesion, migration and proliferation of vascular smooth muscle cells (VSMC) induced by PDGF.METHODS:
?_3 integrin gene expression was detected by RT-PCR. After ?_3 integrin extracellular domain was blocked, VSMC adhesion, migration and proliferation were measured by adhesion assay, a wound-culture model and [~3H]-TdR incorporation, respectively.RESULTS:
After the interaction between ?_3 integrin and extracellular matrix was blocked, VSMC proliferation was inhibited in some degree and the rate of [~3H]-TdR incorporation into VSMC decreased 39%. The cell adhesion and migration were significantly inhibited when 10 mg/L anti-?_3 integrin antibody was added (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
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